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CDS information : Spino_00110

Location

Organism	Saccharopolyspora spinosa
Strain	NRRL 18538
Entry name	Spinosyn
Contig	AY007564
Start / Stop / Direction	14,785 / 13,592 / - [in whole cluster] 14,785 / 13,592 / - [in contig]
Location	complement(13592..14785) [in whole cluster] complement(13592..14785) [in contig]
Type	CDS
Length	1,194 bp (397 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	rhamnose C-3' O-methyltransferase
Product (GenBank)	probable O-methyltransferase
Gene
Gene (GenBank)	spnK
EC number	2.1.1.-
Keyword	tri-O-methylrhamnose
Note
Note (GenBank)	probably involved in rhamnose O-methylation
Reference	ACC Q9ALN2 PmId [11358695] Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa. (Chem Biol. , 2001) [11386361] A cluster of genes for the biosynthesis of spinosyns, novel macrolide insect control agents produced by Saccharopolyspora spinosa. (Antonie Van Leeuwenhoek. , 2000) [20158237] Biosynthesis of spinosyn in Saccharopolyspora spinosa: synthesis of permethylated rhamnose and characterization of the functions of SpnH, SpnI, and SpnK. (J Am Chem Soc. , 2010)

Sequence

>rhamnose C-3' O-methyltransferase [probable O-methyltransferase]
MSTTHEIETVERIILAAGSSAASLADLTTELGLARIAPVLIDEILFRAEPAPDIERTEVA
VQITHRGETVDFVLTLQSGELIKAEQRPVGDVPLRIGYELTDLIAELFGPGAPRAVGARS
TNFLRTTTSGSIPGPSELSDGFQAISAVVAGCGHRRPDLNLLASHYRTDKWGGLHWFTPL
YERHLGEFRDRPVRILEIGVGGYNFDGGGGESLKMWKRYFHRGLVFGMDVFDKSFLDQQR
LCTVRADQSKPEELAAVDDKYGPFDIIIDDGSHINGHVRTSLETLFPRLRSGGVYVIEDL
WTTYAPGFGGQAQCPAAPGTTVSLLKNLLEGVQHEEQPHAGSYEPSYLERNLVGLHTYHN
IAFLEKGVNAEGGVPAWVPRSLDDILHLADVNSAEDE

>rhamnose C-3' O-methyltransferase [probable O-methyltransferase]
ATGTCCACAACGCACGAGATCGAAACCGTGGAACGCATCATCCTCGCCGCCGGATCCAGT
GCGGCGAGCCTGGCCGACCTGACCACCGAACTCGGACTCGCCAGGATCGCACCCGTGCTG
ATCGACGAGATCCTCTTCCGCGCGGAACCGGCCCCCGACATCGAACGGACCGAGGTCGCG
GTCCAGATCACCCACCGAGGCGAGACCGTTGACTTCGTCCTGACGCTACAGTCCGGTGAG
CTGATCAAGGCCGAGCAACGACCGGTCGGAGACGTCCCGCTGCGGATCGGTTACGAGCTC
ACCGATCTCATCGCCGAGTTGTTCGGCCCAGGAGCTCCCAGGGCCGTCGGCGCCCGGAGC
ACCAACTTCCTCCGAACCACCACATCCGGTTCGATACCCGGTCCGTCGGAACTGTCCGAT
GGCTTCCAGGCCATCTCCGCAGTGGTCGCCGGCTGCGGGCACCGACGTCCCGACCTCAAC
TTGCTCGCCTCCCACTACCGCACGGACAAGTGGGGCGGCCTGCACTGGTTCACCCCGCTA
TACGAGCGACACCTCGGCGAGTTCCGTGATCGCCCGGTGCGCATCCTGGAGATCGGTGTC
GGTGGCTACAACTTCGACGGTGGCGGCGGCGAATCCCTGAAGATGTGGAAGCGCTACTTC
CACCGCGGCCTCGTGTTCGGGATGGACGTTTTCGACAAGTCCTTCCTCGACCAGCAGAGG
CTCTGCACCGTCCGCGCCGACCAGAGCAAGCCCGAGGAGCTGGCCGCCGTTGACGACAAG
TACGGACCGTTCGACATCATCATCGACGATGGCAGCCACATCAACGGACACGTGCGCACA
TCCCTGGAAACGCTGTTCCCCCGGTTGCGCAGCGGTGGCGTATACGTGATCGAGGATCTG
TGGACGACCTATGCTCCCGGATTCGGCGGGCAGGCGCAGTGCCCGGCCGCACCCGGCACC
ACGGTCAGCCTGCTCAAGAACCTGTTGGAAGGCGTTCAGCACGAGGAGCAGCCGCATGCG
GGCTCGTACGAGCCGAGCTACCTGGAACGCAATTTGGTCGGCCTCCACACCTACCACAAC
ATCGCGTTCCTGGAGAAAGGCGTCAACGCCGAAGGCGGCGTTCCTGCTTGGGTGCCAAGG
AGTCTGGACGACATATTGCACCTGGCCGACGTGAACAGCGCGGAGGACGAGTGA

Feature

BLASTP Database:UniProtKB:2010_04	show BLAST table
InterPro	No significant hit
SignalP	No significant hit
TMHMM	No significant hit

Spino_00100 Spino_00120

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