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CDS information : Spino_00140


close this sectionLocation

Organism
StrainNRRL 18538
Entry nameSpinosyn
Contig
Start / Stop / Direction18,501 / 17,749 / - [in whole cluster]
18,501 / 17,749 / - [in contig]
Locationcomplement(17749..18501) [in whole cluster]
complement(17749..18501) [in contig]
TypeCDS
Length753 bp (250 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductL-rhamnose C-4' O-methyltransferase
Product (GenBank)probable O-methyltransferase
Gene
Gene (GenBank)spnH
EC number2.1.1.-
Keyword
  • tri-O-methylrhamnose
Note
Note (GenBank)
  • involved in rhamnose O-methylation
Reference
ACC
PmId
[11358695] Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa. (Chem Biol. , 2001)
[11386361] A cluster of genes for the biosynthesis of spinosyns, novel macrolide insect control agents produced by Saccharopolyspora spinosa. (Antonie Van Leeuwenhoek. , 2000)
[18704529] SpnH from Saccharopolyspora spinosa encodes a rhamnosyl 4'-O-methyltransferase for biosynthesis of the insecticidal macrolide, spinosyn A. (J Ind Microbiol Biotechnol. , 2008)
[20158237] Biosynthesis of spinosyn in Saccharopolyspora spinosa: synthesis of permethylated rhamnose and characterization of the functions of SpnH, SpnI, and SpnK. (J Am Chem Soc. , 2010)

close this sectionSequence

selected fasta
>L-rhamnose C-4' O-methyltransferase [probable O-methyltransferase]
MPSQNALYLDLLKKVLTNTIYSDRPHPNAWQDNTDYRQAARAKGTDWPTVAHTMIGLERL
DNLQHCVEAVLADGVPGDFAETGVWRGGACIFMRAVLQAFGDTGRTVWVVDSFQGMPESS
AQDHQADQAMALHEYNDVLGVSLETVRQNFARYGLLDEQVRFLPGWFRDTLPTAPIQELA
VLRLDGDLYESTMDSLRNLYPKLSPGGFVIIDDYFLPSCQDAVKGFRAELGITEPIHDID
GTGAYWRRSW
selected fasta
>L-rhamnose C-4' O-methyltransferase [probable O-methyltransferase]
ATGCCCTCCCAGAACGCGCTGTACCTGGACCTGCTCAAGAAGGTACTCACCAACACGATT
TACAGTGATCGGCCGCATCCGAACGCCTGGCAGGACAACACCGACTACAGGCAGGCCGCT
CGGGCCAAAGGCACGGACTGGCCAACTGTCGCGCACACGATGATCGGTCTGGAGCGGCTG
GACAACCTCCAGCACTGCGTGGAAGCCGTGCTCGCAGACGGTGTTCCCGGGGATTTCGCC
GAGACCGGTGTCTGGCGGGGCGGCGCATGCATCTTCATGCGCGCGGTTCTCCAGGCATTC
GGAGATACCGGACGTACCGTCTGGGTAGTGGATTCCTTCCAGGGAATGCCGGAAAGCTCT
GCGCAAGACCACCAAGCGGACCAGGCTATGGCGCTGCACGAGTACAACGACGTGCTTGGC
GTATCGCTTGAGACCGTCCGGCAGAACTTCGCCCGCTACGGGCTGCTCGACGAACAGGTC
AGGTTCCTCCCCGGCTGGTTCCGGGACACCTTGCCCACCGCCCCCATCCAGGAACTCGCC
GTGCTACGACTCGACGGCGACCTCTACGAATCCACAATGGACTCATTGCGGAACCTGTAC
CCGAAGCTCTCGCCGGGCGGATTCGTCATCATCGACGACTATTTTCTGCCGTCCTGCCAG
GACGCGGTGAAGGGGTTCCGCGCGGAACTCGGGATCACGGAACCCATCCACGACATCGAC
GGCACGGGCGCCTACTGGCGCCGCAGCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR008884 Macrocin-O-methyltransferase (Family)
 [3-249]  4.10000000000004e-130 PF05711
PF05711   TylF
SignalP No significant hit
TMHMM No significant hit