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CDS information : Spino_00150

Location

Organism	Saccharopolyspora spinosa
Strain	NRRL 18538
Entry name	Spinosyn
Contig	AY007564
Start / Stop / Direction	19,713 / 18,541 / - [in whole cluster] 19,713 / 18,541 / - [in contig]
Location	complement(18541..19713) [in whole cluster] complement(18541..19713) [in contig]
Type	CDS
Length	1,173 bp (390 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	glycosyltransferase
Product (GenBank)	probable NDP-rhamnosyltransferase
Gene
Gene (GenBank)	spnG
EC number
Keyword	L-rhamnose
Note
Note (GenBank)	involved in rhamnose addition
Reference	ACC Q9ALM8 PmId [11358695] Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa. (Chem Biol. , 2001) [11386361] A cluster of genes for the biosynthesis of spinosyns, novel macrolide insect control agents produced by Saccharopolyspora spinosa. (Antonie Van Leeuwenhoek. , 2000) [19126547] Functional characterization and substrate specificity of spinosyn rhamnosyltransferase by in vitro reconstitution of spinosyn biosynthetic enzymes. (J Biol Chem. , 2009) [21544146] Enzyme-catalysed [4+2] cycloaddition is a key step in the biosynthesis of spinosyn A. (Nature. , 2011)

Sequence

>glycosyltransferase [probable NDP-rhamnosyltransferase]
MRVLVVPLPYPTHLMAMVPLCWALQASGHEVLIAAPPELQATAHGAGLTTAGIRGNDRTG
DTGGTTQLRFPNPAFGQRDTEAGRQLWEQTASNVAQSSLDQLPEYLRLAEAWRPSVLLVD
VCALIGRVLGGLLDLPVVLHRWGVDPTAGPFSDRAHELLDPVCRHHGLTGLPTPELILDP
CPPSLQASDAPQGAPVQYVPYNGSGAFPAWGAARTSARRVCICMGRMVLNATGPAPLLRA
VAAATELPGVEAVIAVPPEHRALLTDLPDNARIAESVPLNLFLRTCELVICAGGSGTAFT
ATRLGIPQLVLPQYFDQFDYARNLAAAGAGICLPDEQAQSDHEQFTDSIATVLGDTGFAS
AAIKLSDEITAMPHPAALVRTLENTAAIRA

>glycosyltransferase [probable NDP-rhamnosyltransferase]
ATGCGCGTACTCGTCGTTCCCTTGCCCTATCCGACGCATCTCATGGCAATGGTGCCGCTG
TGCTGGGCGCTGCAAGCATCCGGGCACGAGGTCCTGATCGCCGCACCACCAGAGCTGCAG
GCGACCGCGCATGGTGCAGGTCTCACCACGGCCGGGATCCGCGGGAACGACAGGACCGGC
GATACGGGTGGAACCACGCAGCTGCGCTTTCCCAATCCGGCGTTCGGTCAGCGCGACACC
GAGGCAGGCCGGCAACTGTGGGAGCAGACCGCGTCCAATGTCGCGCAAAGCTCGCTCGAT
CAGCTCCCCGAATACCTTCGACTGGCCGAGGCCTGGCGACCGTCAGTGCTGTTGGTCGAC
GTCTGCGCGCTGATCGGCCGGGTGCTCGGCGGATTGCTCGACCTGCCGGTCGTGCTGCAC
CGCTGGGGAGTCGACCCCACCGCAGGCCCCTTCAGCGATCGAGCCCACGAGTTGCTTGAC
CCGGTGTGCCGGCACCACGGACTGACCGGCCTGCCCACTCCCGAGCTCATCCTCGATCCC
TGTCCGCCGAGCCTGCAAGCAAGCGACGCGCCGCAAGGCGCACCGGTCCAGTACGTGCCG
TACAACGGAAGCGGCGCATTCCCGGCATGGGGCGCGGCGCGCACCTCAGCACGGCGGGTC
TGCATCTGCATGGGCCGCATGGTGCTGAACGCCACCGGGCCGGCTCCGCTGCTGCGCGCA
GTAGCGGCTGCCACCGAGTTGCCCGGCGTCGAGGCCGTGATCGCCGTTCCCCCCGAGCAC
CGGGCACTTCTCACCGACCTACCCGACAACGCCCGGATCGCCGAATCGGTCCCGCTCAAC
CTGTTCCTGCGTACCTGCGAGCTGGTCATCTGCGCGGGCGGCTCGGGAACGGCATTCACC
GCGACCCGACTCGGCATCCCGCAACTCGTGCTTCCCCAGTACTTCGACCAGTTCGACTAC
GCGCGCAACCTCGCCGCTGCCGGGGCGGGCATCTGCTTGCCGGATGAGCAGGCCCAGTCC
GACCACGAACAGTTCACCGACTCAATCGCAACGGTGCTCGGCGACACCGGCTTCGCTTCT
GCGGCAATCAAACTCAGCGACGAGATCACGGCCATGCCCCATCCCGCCGCGCTGGTGCGG
ACACTGGAGAACACTGCGGCCATCCGTGCCTGA

Feature

BLASTP Database:UniProtKB:2010_04	show BLAST table
InterPro Database:interpro:26.0	IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28 IPR010610 Protein of unknown function DUF1205 (Domain) PF06722 DUF1205
SignalP	Bacteria, Gram-negative Eukaryota Bacteria, Gram-positive
TMHMM	No significant hit

Spino_00140 Spino_00160

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