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CDS information : Stref_00090


close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction9,225 / 8,890 / - [in whole cluster]
9,225 / 8,890 / - [in contig]
Locationcomplement(8890..9225) [in whole cluster]
complement(8890..9225) [in contig]
TypeCDS
Length336 bp (111 aa)
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close this sectionAnnotation

Category3.3 modification reduction
Productputative C-12 oxygenase
Product (GenBank)monooxygenase
Gene
Gene (GenBank)stfOI
EC number
Keyword
  • anthraquinone
Note
Note (GenBank)
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
Related Reference
ACC
O54259
NITE
Nogl_00350
PmId
[19255477] Expression, purification and crystallization of the cofactor-independent monooxygenase SnoaB from the nogalamycin biosynthetic pathway. (Acta Crystallogr Sect F Struct Biol Cryst Commun. , 2009)
[20052967] Crystal structure of the cofactor-independent monooxygenase SnoaB from Streptomyces nogalater: implications for the reaction mechanism. (Biochemistry. , 2010)
ACC
Q8VWB4
PmId
[12399480] Expression, purification, and characterization of AknX anthrone oxygenase, which is involved in aklavinone biosynthesis in Streptomyces galilaeus. (J Bacteriol. , 2002)

close this sectionSequence

selected fasta
>putative C-12 oxygenase [monooxygenase]
MPTHKYDATDSGIVTFVNQFTVHSSPEEFEKIFAEVSEFMAEQPGFIQYTLSRSIDEDKQ
DRYINIALWEDAQSWRNAVAHPGFQDHAKEIRARTTNVGELYAPRQSFSVK
selected fasta
>putative C-12 oxygenase [monooxygenase]
ATGCCGACGCACAAGTACGACGCCACCGACAGCGGAATCGTCACCTTCGTGAACCAGTTC
ACCGTGCACAGCTCACCGGAGGAATTCGAGAAGATATTCGCCGAGGTCTCCGAATTCATG
GCCGAGCAGCCGGGATTCATCCAGTACACGCTCTCCCGGAGCATCGACGAGGACAAGCAG
GACCGCTACATCAACATCGCACTGTGGGAGGACGCCCAATCCTGGAGGAACGCCGTAGCG
CACCCGGGTTTCCAGGACCACGCCAAGGAGATCCGCGCCCGGACGACGAACGTGGGGGAG
CTCTACGCCCCTCGGCAGTCGTTCTCGGTGAAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR007138 Antibiotic biosynthesis monooxygenase (Domain)
 [13-89]  1.1e-19 PF03992
PF03992   ABM
IPR011008 Dimeric alpha-beta barrel (Domain)
 [4-106]  2.90000354677981e-19 SSF54909
SSF54909   Dimer_A_B_barrel
SignalP No significant hit
TMHMM No significant hit