close all open/close all

CDS information : Stref_00130


close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction11,796 / 12,668 / + [in whole cluster]
11,796 / 12,668 / + [in contig]
Location11796..12668 [in whole cluster]
11796..12668 [in contig]
TypeCDS
Length873 bp (290 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative transcriptional regulator
Product (GenBank)SARP family regulator
Gene
Gene (GenBank)stfRI
EC number
Keyword
  • SARP family
Note
Note (GenBank)
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
Related Reference
ACC
P25047
NITE
Adria_00210
PmId
[1729206] Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius. (J Bacteriol. , 1992)
[7868593] Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. (J Bacteriol. , 1995)
[8971703] Purification and characterization of the DNA-binding protein DnrI, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius. (Mol Microbiol. , 1996)
[16545946] Feedback regulation of doxorubicin biosynthesis in Streptomyces peucetius. (Res Microbiol. , 2006)
[20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010)
[20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010)

close this sectionSequence

selected fasta
>putative transcriptional regulator [SARP family regulator]
MQIKTLGPLVAQENQVSLVPSAGKPRQVLSLLALNANRVISVGTLIEEIWGNNPPRSSAT
TLQTYIFQLRRNLDSALDRNDGLTAKDILVTQHNGYLLRARPGDIDVHEFERLAGEARSA
AAAGDDAAAADLFTEALSLWDGPALVDVQAGRILEPEIMRLTEARLAAVEGRLAARLRMG
RHAELLSELTVLTMKYPMHENLCAQFMIALHRSGRSWNALEAYRRLRDTMIEELGLEPSA
RLRRLHQAVLAGDPVLDPSAAADGLDFERQAVTGPPPMRPTSAASPVARV
selected fasta
>putative transcriptional regulator [SARP family regulator]
GTGCAGATAAAGACTCTCGGTCCCCTCGTCGCACAGGAAAACCAGGTCTCCTTGGTGCCC
AGCGCCGGCAAACCGAGGCAGGTCCTCTCACTGCTCGCCCTCAACGCCAACCGCGTCATC
AGCGTGGGAACACTCATCGAGGAAATATGGGGAAACAACCCCCCACGCAGTTCGGCGACC
ACGCTCCAGACCTACATTTTCCAACTGCGCAGAAATCTGGATTCCGCGCTCGACCGGAAC
GACGGGCTGACGGCCAAGGACATCCTGGTCACTCAGCACAACGGGTATCTGCTGCGGGCA
CGGCCCGGAGACATCGATGTCCATGAATTCGAGCGCCTCGCCGGCGAGGCCCGCTCGGCG
GCGGCCGCGGGTGACGACGCGGCGGCCGCGGACCTGTTCACCGAGGCGCTGTCCCTGTGG
GACGGCCCGGCTCTGGTCGACGTACAGGCCGGACGGATCCTCGAACCGGAGATCATGAGA
CTGACGGAGGCCAGGCTTGCCGCGGTGGAGGGACGCCTGGCGGCCCGGCTGCGGATGGGC
CGCCACGCGGAGCTGCTCAGCGAGCTGACCGTCCTGACCATGAAGTACCCGATGCACGAG
AACCTGTGCGCCCAGTTCATGATCGCCCTGCACCGCTCCGGCCGGTCGTGGAACGCCCTG
GAGGCGTACCGCAGGCTGCGGGACACGATGATCGAGGAGCTGGGCTTGGAGCCGTCGGCC
CGGTTACGCCGGCTGCACCAGGCCGTACTCGCCGGTGATCCGGTGCTCGACCCGTCCGCG
GCCGCGGACGGGCTGGACTTCGAACGTCAGGCCGTCACCGGCCCGCCGCCGATGCGTCCG
ACGAGCGCCGCGTCTCCGGTCGCACGCGTATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001867 Signal transduction response regulator, C-terminal (Domain)
 [19-98]  2.2e-18 PF00486
PF00486   Trans_reg_C
 [13-98]  7.39999967908914e-08 SM00862
SM00862   Trans_reg_C
IPR005158 Bacterial transcriptional activator domain (Domain)
 [105-250]  4.39999999999997e-50 PF03704
PF03704   BTAD
 [105-250]  6.00001679190159e-51 SM01043
SM01043   BTAD
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
 [24-100]  5.69999999999999e-10 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR016032 Signal transduction response regulator, C-terminal effector (Domain)
 [1-99]  1.20000117458134e-13 SSF46894
SSF46894   Bipartite_resp_reg_C-effector
SignalP No significant hit
TMHMM No significant hit