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CDS information : Stref_00260


close this sectionLocation

Organism
StrainNRRL 3193 (=NBRC 13446)
Entry nameSteffimycin
Contig
Start / Stop / Direction26,916 / 25,654 / - [in whole cluster]
26,916 / 25,654 / - [in contig]
Locationcomplement(25654..26916) [in whole cluster]
complement(25654..26916) [in contig]
TypeCDS
Length1,263 bp (420 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productglycosyltransferase
Product (GenBank)glycosyl transferase
Gene
Gene (GenBank)stfG
EC number
Keyword
  • L-rhamnose
Note
Note (GenBank)
Reference
ACC
PmId
[16751529] Isolation, characterization, and heterologous expression of the biosynthesis gene cluster for the antitumor anthracycline steffimycin. (Appl Environ Microbiol. , 2006)
[18224649] Glycosylated derivatives of steffimycin: insights into the role of the sugar moieties for the biological activity. (Chembiochem. , 2008)
Related Reference
ACC
Q9L4U6
NITE
Acla_00210
PmId
[15911373] AknT is an activating protein for the glycosyltransferase AknS in L-aminodeoxysugar transfer to the aglycone of aclacinomycin A. (Chem Biol. , 2005)
[17685523] Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A. (J Am Chem Soc. , 2007)
ACC
Q9ZGH7
NITE
Pikro_00090
PmId
[15161264] Characterization of the glycosyltransferase activity of desVII: analysis of and implications for the biosynthesis of macrolide antibiotics. (J Am Chem Soc. , 2004)
[15358425] New olivosyl derivatives of methymycin/pikromycin from an engineered strain of Streptomyces venezuelae. (FEMS Microbiol Lett. , 2004)
[20695498] Characterization of glycosyltransferase DesVII and its auxiliary partner protein DesVIII in the methymycin/picromycin biosynthetic pathway. (Biochemistry. , 2010)
[23770075] Combinatorial biosynthesis and antibacterial evaluation of glycosylated derivatives of 12-membered macrolide antibiotic YC-17. (J Biotechnol. , 2013)
[26552796] In vivo investigation to the macrolide-glycosylating enzyme pair DesVII/DesVIII in Saccharopolyspora erythraea. (Appl Microbiol Biotechnol. , 2016)

close this sectionSequence

selected fasta
>glycosyltransferase [glycosyl transferase]
MRVLMTSLAVEAHFNGAVPLAWALRAAGHEVRVAGQPALTEAITRAGLTAVPVGTDHTHH
ELVRELGAELTGFYRDIDLTGERGDDYLALKGANTLLTATFYAQANNDPMVDELVDYARY
WRPDLVIWEPFTFAGAVAARASGAAHARLLWGPDLFLRVRRAFRDQMERLPRELHDDTLE
EWLTWTLGRFGCDFDEETVVGQWDIDQMPAGIRLPLGRPTVPMRYVPYNGRSVVPAWLRE
PPRRPRICLTLGITSRDMAYPDRISLGELLEAVAELDVEIVATLTAAQRAEVARVPDNTR
VTDFVPMHALLPSCSAVVHHGGAGTWSTAAACGVPQLVIADMWDNVYRARRLADLGAGLF
LPPERLTVAGLRDGLEQLLTKPSFREDAERLRAEMTAEPSPADVVPALEELTRRHRTGRR
selected fasta
>glycosyltransferase [glycosyl transferase]
ATGCGCGTTCTGATGACGTCGCTCGCCGTCGAAGCACACTTCAACGGCGCTGTTCCACTG
GCCTGGGCGCTGCGCGCGGCGGGGCACGAGGTCCGGGTGGCCGGGCAGCCCGCGCTCACC
GAGGCCATCACCCGGGCCGGACTGACCGCGGTGCCCGTGGGCACCGACCACACCCACCAC
GAGCTCGTGCGTGAACTCGGTGCCGAACTCACCGGCTTCTACCGCGACATCGACCTGACG
GGGGAGCGCGGCGACGACTATCTGGCGCTCAAGGGCGCCAACACCCTGCTCACCGCGACG
TTCTACGCCCAGGCCAACAATGACCCGATGGTGGATGAACTGGTCGATTACGCCCGTTAC
TGGCGACCCGACCTGGTGATCTGGGAGCCGTTCACCTTCGCCGGCGCCGTGGCCGCGCGG
GCCAGTGGGGCCGCCCACGCGCGACTGCTGTGGGGGCCGGATCTCTTCCTCCGCGTACGC
CGGGCGTTCCGCGACCAGATGGAACGCCTGCCGCGGGAACTGCACGACGACACCCTCGAG
GAGTGGCTGACCTGGACGCTCGGGCGGTTCGGCTGTGACTTCGACGAGGAGACGGTGGTC
GGCCAGTGGGACATCGACCAGATGCCGGCCGGTATCCGGCTGCCCCTGGGGCGTCCGACG
GTGCCGATGCGTTATGTGCCCTACAACGGCAGGTCGGTCGTCCCCGCATGGCTGCGGGAA
CCGCCGCGTCGACCGCGGATCTGTCTGACGCTGGGCATCACCTCCCGTGACATGGCCTAT
CCGGATCGCATCTCCCTCGGCGAACTGCTCGAAGCCGTCGCCGAGTTGGACGTCGAGATC
GTCGCCACCCTCACCGCGGCGCAACGGGCCGAGGTGGCGCGGGTACCGGACAACACACGT
GTCACGGACTTCGTGCCCATGCACGCCCTGCTGCCGAGCTGCTCGGCCGTGGTCCACCAC
GGCGGCGCGGGCACCTGGTCCACCGCCGCCGCGTGCGGGGTGCCCCAACTCGTGATCGCC
GACATGTGGGACAACGTGTACCGGGCGCGACGGCTGGCAGACCTGGGCGCGGGGCTGTTC
CTGCCGCCGGAGCGACTGACGGTGGCCGGTCTGCGCGACGGCCTGGAGCAGCTGCTGACG
AAGCCGTCCTTCCGGGAGGACGCGGAGCGGCTCCGGGCGGAGATGACCGCCGAACCCAGC
CCCGCCGACGTCGTGCCCGCCCTGGAGGAACTGACCCGGCGCCACCGCACCGGCCGGCGG
TGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
 [4-71]  4.80000000000001e-12 PF03033
PF03033   Glyco_transf_28
IPR010610 Domain of unknown function DUF1205 (Domain)
 [205-300]  1.5e-34 PF06722
PF06722   DUF1205
SignalP
 [1-23]  0.857 Signal
Eukaryota   
TMHMM No significant hit