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CDS information : Vanco_00140


close this sectionLocation

Organism
StrainATCC 19795 (=NBRC 12806)
Entry nameVancomycin
Contig
Start / Stop / Direction40,162 / 41,382 / + [in whole cluster]
40,162 / 41,382 / + [in contig]
Location40162..41382 [in whole cluster]
40162..41382 [in contig]
TypeCDS
Length1,221 bp (406 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative cytochrome P450
Product (GenBank)P450 monooxygenase C
Gene
Gene (GenBank)oxyC
EC number
Keyword
  • 3rd cross-linking
  • AB ring
Note
Note (GenBank)
Reference
ACC
Related Reference
ACC
Q8RN03
PmId
[12207020] Crystal structure of OxyB, a cytochrome P450 implicated in an oxidative phenol coupling reaction during vancomycin biosynthesis. (J Biol Chem. , 2002)
[12888556] Crystal structure of OxyC, a cytochrome P450 implicated in an oxidative C-C coupling reaction during vancomycin biosynthesis. (J Biol Chem. , 2003)
ACC
O87675
NITE
Balhi_00130
PmId
[12404385] The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-The Order of the Cyclization Steps This work was supported by the Deutsche Forschungsgemeinschaft (SFB 323) and by a grant of the EU (MEGATOP, QLK3-1999-00650). R. D. S. gratefully acknowledges the support of a Feodor-Lynen Fellowship granted by the Alexander-von-Humboldt Stiftung. We thank Corina Bihlmaier and Volker Pfeifer for help with transformation and Southern hybridization, J. A. Moss (La Jolla (USA)) for critical comments on the manuscript and Prof. Dr. M. E. Maier and Prof. Dr. H.-P. Fiedler (Tubingen) for generous support. (Angew Chem Int Ed Engl. , 2001)
[16730832] Genetic analysis of the balhimycin (vancomycin-type) oxygenase genes. (J Biotechnol. , 2006)
ACC
R4SZ79
PmId
[24884615] Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis. (BMC Genomics. , 2014)

close this sectionSequence

selected fasta
>putative cytochrome P450 [P450 monooxygenase C]
MGHDIDQVAPLLREPANFQLRTNCDPHEDNFGLRAHGPLVRIVGESSTQLGRDFVWQAHG
YEVVRRILGDHEHFTTRPQFTQSKSGAHVEAQFVGQISTYDPPEHTRLRKMLTPEFTVRR
IRRMEPAIQSLIDDRLDLLEAEGPSADLQGLFADPVGAHALCELLGIPRDDQREFVRRIR
RNADLSRGLKARAADSAAFNRYLDNLLARQRADPDDGLLGMIVRDHGDNVTDEELKGLCT
ALILGGVETVAGMIGFGVLALLDNPGQIELLFESPEKAERVVNELVRYLSPVQAPNPRLA
IKDVVIDGQLIKAGDYVLCSILMANRDEALTPDPDVLDANRAAVSDVGFGHGIHYCVGAA
LARSMLRMAYQTLWRRFPGLRLAVPIEEVKYRSAFVDCPDQVPVTW
selected fasta
>putative cytochrome P450 [P450 monooxygenase C]
ATGGGGCACGATATCGATCAGGTCGCGCCGCTCCTGCGGGAGCCGGCGAACTTTCAGCTG
AGGACGAACTGCGATCCGCACGAGGACAATTTCGGGCTCAGGGCGCACGGCCCTTTGGTG
CGGATAGTCGGCGAATCGTCCACCCAGCTGGGCAGGGACTTCGTCTGGCAGGCCCACGGT
TACGAGGTCGTGCGCAGGATATTGGGTGATCACGAACATTTCACGACGCGGCCGCAATTC
ACCCAGTCGAAATCCGGCGCCCATGTGGAGGCCCAGTTCGTCGGGCAGATCTCCACTTAT
GACCCACCCGAGCACACCCGTTTGCGGAAAATGCTCACGCCGGAGTTCACGGTCCGCCGG
ATCCGCCGGATGGAGCCCGCGATCCAAAGTCTCATCGATGATCGCCTCGATCTGCTGGAG
GCCGAGGGGCCGTCCGCGGACCTCCAGGGCCTGTTCGCCGATCCGGTCGGCGCGCACGCC
CTGTGCGAACTGCTCGGCATCCCGCGCGACGACCAGCGTGAGTTCGTCCGGCGGATCAGG
CGGAACGCCGACCTGAGCCGGGGACTCAAGGCACGGGCCGCCGACAGCGCGGCGTTCAAC
CGGTATTTGGACAACCTCCTCGCCAGGCAGCGCGCCGACCCCGACGACGGTCTCCTCGGC
ATGATCGTGCGGGACCACGGCGACAACGTCACGGACGAGGAACTGAAGGGCCTCTGCACG
GCGCTGATCCTCGGCGGTGTCGAAACCGTCGCCGGGATGATCGGTTTCGGTGTCCTCGCC
CTCCTGGACAATCCCGGACAGATCGAGTTGCTTTTCGAGTCCCCCGAAAAGGCCGAACGC
GTCGTCAACGAACTCGTCCGGTATCTGTCCCCGGTGCAGGCGCCGAATCCGCGTCTCGCC
ATCAAGGACGTGGTCATCGACGGGCAGCTGATCAAGGCGGGGGACTACGTCCTGTGCTCG
ATTCTCATGGCCAACCGGGATGAGGCGCTGACCCCGGATCCCGACGTCCTCGACGCGAAT
CGCGCCGCGGTGTCGGACGTCGGATTCGGGCACGGCATCCATTACTGCGTCGGCGCGGCC
TTGGCCAGATCCATGCTGCGCATGGCTTATCAGACTTTGTGGCGGCGATTCCCGGGACTG
CGGCTGGCAGTGCCCATCGAAGAAGTGAAGTACCGCAGCGCGTTCGTAGATTGCCCTGAT
CAGGTACCGGTCACCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001128 Cytochrome P450 (Family)
 [2-406]  1.1e-93 SSF48264
SSF48264   Cytochrome P450
 [9-406]  2.4e-91 G3DSA:1.10.630.10
G3DSA:1.10.630.10   no description
 [279-377]  1.8e-12 PF00067
PF00067   p450
IPR002397 Cytochrome P450, B-class (Family)
 [100-111]  2e-47 PR00359 [147-163]  2e-47 PR00359 [164-179]  2e-47 PR00359 [201-223]  2e-47 PR00359 [280-291]  2e-47 PR00359 [298-325]  2e-47 PR00359 [326-341]  2e-47 PR00359 [347-356]  2e-47 PR00359 [356-367]  2e-47 PR00359
PR00359   BP450
IPR017972 Cytochrome P450, conserved site (Conserved_site)
 [349-358]  PS00086
PS00086   CYTOCHROME_P450
SignalP No significant hit
TMHMM No significant hit