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CDS information : Vanco_00160


close this sectionLocation

Organism
StrainATCC 19795 (=NBRC 12806)
Entry nameVancomycin
Contig
Start / Stop / Direction42,934 / 44,160 / + [in whole cluster]
42,934 / 44,160 / + [in contig]
Location42934..44160 [in whole cluster]
42934..44160 [in contig]
TypeCDS
Length1,227 bp (408 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productglycosyltransferase
Product (GenBank)glycosyltransferase D
Gene
Gene (GenBank)gtfD
EC number
Keyword
  • L-vancosamine
  • Hpg4
Note
  • This ORF product can also catalyze a reversible reaction.
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference
ACC
PmId
[11294642] Tandem action of glycosyltransferases in the maturation of vancomycin and teicoplanin aglycones: novel glycopeptides. (Biochemistry. , 2001)
[12498883] Incorporation of glucose analogs by GtfE and GtfD from the vancomycin biosynthetic pathway to generate variant glycopeptides. (Chem Biol. , 2002)
[15122882] Crystal structure of vancosaminyltransferase GtfD from the vancomycin biosynthetic pathway: interactions with acceptor and nucleotide ligands. (Biochemistry. , 2004)
[16045364] A systematic investigation of the synthetic utility of glycopeptide glycosyltransferases. (J Am Chem Soc. , 2005)
[16946071] Exploiting the reversibility of natural product glycosyltransferase-catalyzed reactions. (Science. , 2006)
[24954524] Enzymatic glycosylation of vancomycin aglycon: completion of a total synthesis of vancomycin and N- and C-terminus substituent effects of the aglycon substrate. (Org Lett. , 2014)
Related Reference
ACC
P96564
PmId
[9115410] Production of hybrid glycopeptide antibiotics in vitro and in Streptomyces toyocaensis. (Chem Biol. , 1997)
ACC
R4SKH3
PmId
[24884615] Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis. (BMC Genomics. , 2014)

close this sectionSequence

selected fasta
>glycosyltransferase [glycosyltransferase D]
MRVLLSVCGTRGDVEIGVALADRLKALGVQTRMCAPPAAEERLAEVGVPHVPVGLPQHMM
LQEGMPPPPPEEEQRLAAMTVEMQFDAVPGAAEGCAAVVAVGDLAAATGVRSVAEKLGLP
FFYSVPSPVYLASPHLPPAYDEPTTPGVTDIRVLWEERAARFADRYGPTLNRRRAEIGLP
PVEDVFGYGHGERPLLAADPVLAPLQPDVDAVQTGAWLLSDERPLPPELEAFLAAGSPPV
HIGFGSSSGRGIADAAKVAVEAIRAQGRRVILSRGWTELVLPDDRDDCFAIDEVNFQALF
RRVAAVIHHGSAGTEHVATRAGVPQLVIPRNTDQPYFAGRVAALGIGVAHDGPTPTFESL
SAALTTVLAPETRARAEAVAGMVLTDGAAAAADLVLAAVGREKPAVPA
selected fasta
>glycosyltransferase [glycosyltransferase D]
ATGCGTGTGCTGTTGTCGGTGTGCGGAACCCGCGGGGACGTCGAAATCGGGGTGGCGCTG
GCGGACCGATTGAAGGCGCTCGGCGTCCAGACACGGATGTGCGCGCCGCCCGCCGCCGAG
GAGCGGCTGGCGGAGGTCGGGGTGCCGCATGTGCCGGTCGGCCTGCCGCAGCACATGATG
TTGCAGGAGGGCATGCCGCCGCCACCGCCCGAAGAGGAACAGCGGCTGGCGGCCATGACG
GTCGAGATGCAGTTCGACGCCGTCCCCGGGGCCGCCGAAGGGTGTGCCGCGGTGGTGGCG
GTCGGCGATCTGGCCGCCGCGACCGGTGTCCGGTCGGTGGCCGAGAAACTGGGCCTGCCC
TTCTTCTATTCCGTCCCCAGTCCGGTCTATCTGGCGTCCCCGCACCTTCCGCCGGCTTAC
GACGAGCCGACCACGCCGGGCGTGACCGACATCCGGGTGCTGTGGGAGGAGCGGGCCGCC
CGGTTCGCCGACCGGTACGGGCCCACGCTCAACCGTCGCCGGGCCGAGATCGGCCTGCCG
CCGGTGGAGGACGTCTTCGGCTACGGCCACGGTGAGCGGCCCCTGCTGGCGGCGGACCCG
GTACTGGCGCCCCTGCAGCCGGACGTCGACGCGGTGCAGACCGGTGCGTGGCTCCTGTCC
GACGAGCGGCCGCTTCCCCCGGAGCTGGAAGCGTTCCTGGCCGCCGGGTCACCGCCGGTG
CACATCGGTTTCGGCAGCTCGTCCGGCCGCGGGATCGCCGACGCCGCGAAGGTGGCCGTC
GAGGCGATCCGGGCCCAAGGCCGGCGGGTGATCCTCTCGCGGGGGTGGACCGAGCTGGTC
CTGCCCGACGACCGTGACGACTGTTTCGCCATCGACGAGGTGAACTTCCAGGCGCTGTTC
CGCCGGGTGGCCGCCGTCATCCACCACGGCAGCGCGGGTACGGAGCACGTGGCCACGCGG
GCCGGCGTCCCCCAGCTCGTGATCCCCCGGAACACGGACCAGCCGTACTTCGCCGGGCGG
GTGGCCGCGCTGGGGATCGGTGTGGCGCATGACGGCCCGACGCCGACCTTCGAGTCCCTT
TCGGCCGCGCTCACCACGGTGCTCGCGCCGGAGACGCGGGCGCGGGCGGAGGCCGTGGCG
GGCATGGTCCTCACCGACGGGGCGGCGGCGGCCGCGGATCTGGTGCTCGCCGCGGTCGGC
CGGGAGAAGCCGGCCGTTCCCGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR004276 Glycosyl transferase, family 28 (Domain)
 [3-134]  7.1e-27 PF03033
PF03033   Glyco_transf_28
SignalP No significant hit
TMHMM No significant hit