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CDS information : Vanco_00310


close this sectionLocation

Organism
StrainATCC 19795 (=NBRC 12806)
Entry nameVancomycin
Contig
Start / Stop / Direction61,447 / 62,064 / + [in whole cluster]
61,447 / 62,064 / + [in contig]
Location61447..62064 [in whole cluster]
61447..62064 [in contig]
TypeCDS
Length618 bp (205 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative dTDP-3-amino-4-keto-2,3,6-trideoxy-3-C-methyl-D-glucose 5-epimerase
Product (GenBank)dTDP-4-keto-6-deoxyglucose 3,5-epimerase
Gene
Gene (GenBank)vcaD
EC number5.1.3.-
Keyword
  • L-vancosamine
Note
Note (GenBank)
Reference
ACC
Related Reference
ACC
R4SKI5
PmId
[24884615] Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis. (BMC Genomics. , 2014)
ACC
O52806
NITE
Chlor_00270
PmId
[11035791] Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. (Proc Natl Acad Sci U S A. , 2000)
[12077451] Purification, crystallization and preliminary structural studies of dTDP-4-keto-6-deoxy-glucose-5-epimerase (EvaD) from Amycolatopsis orientalis, the fourth enzyme in the dTDP-L-epivancosamine biosynthetic pathway. (Acta Crystallogr D Biol Crystallogr. , 2002)
[15159413] The position of a key tyrosine in dTDP-4-Keto-6-deoxy-D-glucose-5-epimerase (EvaD) alters the substrate profile for this RmlC-like enzyme. (J Biol Chem. , 2004)

close this sectionSequence

selected fasta
>putative dTDP-3-amino-4-keto-2,3,6-trideoxy-3-C-methyl-D-glucose 5-epimerase [dTDP-4-keto-6-deoxyglucose 3,5-epimerase]
MQARKLAVEGAIEFTPRVFPDDRGLFVSPFQEEAFAEARGGPLFRVAQTNHSMSKRGVVR
GVHYTMTPPGIAKYVYCARGSVLDIVVDIRVGSPTFGRWDAVLLDQRDHRAMYFPVGVGH
AFVALEDDSVMWYMLSASYVPRNELALSALDPALDLPIDASVEPILSDRDKVAITLAEAE
RQGLLPDYATSLELERRLSEVPLTA
selected fasta
>putative dTDP-3-amino-4-keto-2,3,6-trideoxy-3-C-methyl-D-glucose 5-epimerase [dTDP-4-keto-6-deoxyglucose 3,5-epimerase]
GTGCAAGCACGCAAACTGGCCGTCGAGGGCGCGATCGAGTTCACTCCCCGGGTCTTCCCC
GACGACCGCGGCCTGTTCGTCTCGCCGTTCCAGGAAGAGGCCTTCGCCGAGGCCCGCGGC
GGTCCGCTCTTCCGGGTGGCGCAGACGAACCACAGCATGTCCAAACGCGGCGTGGTGCGG
GGAGTCCACTACACCATGACGCCGCCCGGGATCGCGAAGTACGTCTACTGCGCCCGCGGC
AGTGTCCTGGACATCGTGGTCGACATCCGGGTCGGTTCGCCGACGTTCGGCCGGTGGGAC
GCGGTCCTGCTCGACCAGCGGGACCACCGGGCGATGTACTTCCCGGTCGGCGTCGGCCAC
GCGTTCGTGGCGCTCGAAGACGACAGCGTCATGTGGTACATGCTCTCCGCGAGCTACGTA
CCGCGGAACGAACTCGCCCTCTCGGCGCTGGATCCCGCGCTGGACCTGCCCATCGACGCG
AGTGTCGAGCCGATCCTGTCCGATCGGGACAAGGTGGCCATCACCCTCGCCGAGGCCGAG
CGGCAGGGGCTGCTGCCGGATTACGCCACCAGCCTGGAACTCGAGCGACGGCTTTCCGAA
GTCCCGCTCACCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family)
 [1-183]  PD001462
PD001462   dTDP_sugar_isom
 [4-178]  4.10000000000004e-58 PF00908
PF00908   dTDP_sugar_isom
IPR011051 RmlC-like cupin domain (Domain)
 [1-183]  1.3000049540733e-66 SSF51182
SSF51182   RmlC_like_cupin
IPR014710 RmlC-like jelly roll fold (Domain)
 [1-189]  1.2e-59 G3DSA:2.60.120.10
G3DSA:2.60.120.10   RmlC-like_jellyroll
SignalP No significant hit
TMHMM No significant hit