close all open/close all

CDS information : Vanco_00320


close this sectionLocation

Organism
StrainATCC 19795 (=NBRC 12806)
Entry nameVancomycin
Contig
Start / Stop / Direction62,154 / 63,272 / + [in whole cluster]
62,154 / 63,272 / + [in contig]
Location62154..63272 [in whole cluster]
62154..63272 [in contig]
TypeCDS
Length1,119 bp (372 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)dihydroxyphenylacetic acid synthase
Gene
Gene (GenBank)dpgA
EC number
Keyword
  • type III PKS
  • Dpg
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
Reference
ACC
Related Reference
ACC
Q939X3
NITE
Balhi_00320
PmId
[11495926] A polyketide synthase in glycopeptide biosynthesis: the biosynthesis of the non-proteinogenic amino acid (S)-3,5-dihydroxyphenylglycine. (J Biol Chem. , 2001)
ACC
O52795
NITE
Chlor_00280
PmId
[11752437] Glycopeptide antibiotic biosynthesis: enzymatic assembly of the dedicated amino acid monomer (S)-3,5-dihydroxyphenylglycine. (Proc Natl Acad Sci U S A. , 2001)
[12240210] Biosynthesis of the vancomycin group of antibiotics: characterisation of a type III polyketide synthase in the pathway to (S)-3,5-dihydroxyphenylglycine. (Chem Commun (Camb). , 2001)
[14744141] Role of the active site cysteine of DpgA, a bacterial type III polyketide synthase. (Biochemistry. , 2004)
[22492619] Chain elongation and cyclization in type III PKS DpgA. (Chembiochem. , 2012)
ACC
R4SN05
PmId
[24884615] Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis. (BMC Genomics. , 2014)

close this sectionPKS/NRPS Module

C1
CHS56..370

close this sectionSequence

selected fasta
>polyketide synthase [dihydroxyphenylacetic acid synthase]
MGMDVSMTTGIELTEELSVLNGLTEITRFAGVGTAVSETSYSQTELLDILDVEDPKIRSV
FLNSAIDRRFLTLPPENPGGGRLAEPQGDLLDKHKKIAVDMGCRALEACLKSAGATLSDL
RHLCCVTSTGFLTPGLSALIIREMGIDPHCSRSDIVGMGCNAGLNALNVVSGWSAAHPGE
LGVVLCSEACSAAYALDGTMRTAVVNSLFGDGSAALAVISGDGRVAGPRVLKFASYIITD
AVDAMRYDWDRDQDRFSFFLDPQIPYVVGAHAEIVVDRLLSGTGLRRSDIGHWLVHSGGK
KVVDAVVVNLGLSRHDVRHTTGVLRDYGNLSSGSFLFSYERLSEEDVTRPGDYGVLMTMG
PGSTIEMALIQW
selected fasta
>polyketide synthase [dihydroxyphenylacetic acid synthase]
TTGGGGATGGATGTATCGATGACTACCGGAATCGAACTCACCGAAGAGCTGTCCGTCCTC
AATGGACTGACCGAGATCACCAGATTCGCCGGCGTCGGGACCGCGGTCTCCGAGACGTCC
TATTCGCAGACCGAGCTGTTGGACATCCTCGACGTCGAGGATCCCAAGATCCGATCGGTC
TTCCTGAACAGCGCGATCGACCGGCGTTTCCTCACCCTGCCACCGGAGAACCCCGGCGGG
GGACGTCTGGCGGAACCGCAGGGCGACCTCCTCGACAAGCACAAGAAGATCGCCGTCGAC
ATGGGCTGCCGCGCGCTCGAAGCGTGCCTGAAGTCGGCGGGGGCGACGCTTTCGGACCTG
CGTCACCTGTGCTGCGTCACCTCGACCGGGTTCCTGACCCCCGGCCTGAGCGCGCTGATC
ATCCGCGAGATGGGCATCGACCCGCACTGCAGCCGCTCGGACATCGTGGGCATGGGCTGC
AACGCCGGACTGAACGCGCTGAACGTGGTCTCCGGCTGGTCCGCGGCCCATCCGGGCGAA
CTCGGCGTCGTCCTGTGCAGCGAGGCGTGTTCCGCCGCCTACGCCCTGGACGGCACCATG
CGGACCGCGGTGGTCAACAGCCTCTTCGGCGACGGCTCCGCGGCCCTCGCCGTCATCTCG
GGCGACGGCCGGGTGGCCGGCCCGCGTGTCCTCAAGTTCGCGAGTTACATCATCACCGAC
GCGGTCGACGCGATGCGGTACGACTGGGACCGCGACCAGGACCGCTTCAGCTTCTTCCTC
GACCCGCAGATCCCCTACGTGGTCGGTGCGCACGCCGAGATCGTCGTCGACCGGCTCCTG
TCCGGGACGGGACTGCGCCGCAGCGACATCGGCCACTGGCTGGTGCATTCCGGCGGCAAG
AAGGTGGTCGACGCCGTCGTCGTCAATCTGGGCCTGAGCAGGCACGACGTCCGGCACACC
ACCGGGGTGCTCCGTGACTACGGGAACCTGTCCAGCGGCTCCTTCCTCTTCTCCTACGAG
CGACTGTCCGAAGAGGACGTCACCCGCCCCGGAGACTACGGCGTGCTCATGACCATGGGG
CCCGGGTCCACGATCGAAATGGCGCTGATCCAATGGTGA
[1] CHS56..370
[1] CHS166..1110

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09
show BLAST table
InterPro
Database:interpro:38.0
IPR001099 Chalcone/stilbene synthase, N-terminal (Domain)
 [56-218]  2.8e-14 PF00195
PF00195   Chal_sti_synt_N
IPR011141 Polyketide synthase, type III (Family)
 [1-372]  8.5e-139 PIRSF000451
PIRSF000451   Type III polyketide synthase
IPR012328 Chalcone/stilbene synthase, C-terminal (Domain)
 [257-370]  3.6e-14 PF02797
PF02797   Chal_sti_synt_C
IPR016038 Thiolase-like, subgroup (Domain)
 [30-222]  3.9e-38 G3DSA:3.40.47.10 [229-372]  2.4e-32 G3DSA:3.40.47.10
G3DSA:3.40.47.10   no description
IPR016039 Thiolase-like (Domain)
 [31-372]  3.5e-44 SSF53901
SSF53901   Thiolase-like
SignalP No significant hit
TMHMM No significant hit