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DoBISCUIT (Database of BIoSynthesis cluster CUrated and InTegrated)

Secondary metabolites produced by actinomycetes are important as lead-compounds and/or candidates for drug development. Polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs) have been attracting much attention for their roles in constructing complex compounds. Secondary metabolite biosynthesis gene clusters are often used in biotechnological applications such as heterologous expression and combinatorial biosynthesis. Many scientific papers are published describing biosynthesis gene clusters every year, but information about a particular gene cluster is often dispersed in many references and is not described in a comprehensive manner.

We constructed a literature-based database about known PKS and NRPS gene clusters. The database consists of biosynthesis cluster information, search menu, retrieve information menu and KS/A domain sequence menu. Our database enables easy access to a comprehensive information related to biosynthesis clusters and browsing standardized up-to-date gene descriptions. Our database will thus serve as a useful reference for the analysis of secondary metabolites. biosynthetic gene clusters.

open this section0.Interface overview

open this section1.Top page

open this section2.Compound list

open this section3.Cluster information

open this section4.CDS list

open this section5.CDS information

open this section6.Simple text search

close this section7.Advanced text search

Advanced text search menu is linked from middle-left panel of Top page. Users can search within DoBISCUIT by entering the search words, specifying the target fields and select the target clusters. Spaces between words are regarded as 'AND' search term. To search for exact phrase, enclose the phrase in double quotation.


Search result will be displayed as the list of clusters containing the search term. You can follow the hyperlink to the biosynthesis cluster page or check a box you want to browse and go to CDS tab to browse the CDSs.

open this section8.Module search

open this section9.BLAST search

open this section10.Category list

open this section11.Get sequence

open this section12.Data download

open this section13.PCR amplified sequences

open this section14.Novelty chart

open this section15.PCR sequence BLAST

open this sectionXX.Other tips implemented in DoBISCUIT

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