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DoBISCUIT (Database of BIoSynthesis cluster CUrated and InTegrated)

Secondary metabolites produced by actinomycetes are important as lead-compounds and/or candidates for drug development. Polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs) have been attracting much attention for their roles in constructing complex compounds. Secondary metabolite biosynthesis gene clusters are often used in biotechnological applications such as heterologous expression and combinatorial biosynthesis. Many scientific papers are published describing biosynthesis gene clusters every year, but information about a particular gene cluster is often dispersed in many references and is not described in a comprehensive manner.

We constructed a literature-based database about known PKS and NRPS gene clusters. The database consists of biosynthesis cluster information, search menu, retrieve information menu and KS/A domain sequence menu. Our database enables easy access to a comprehensive information related to biosynthesis clusters and browsing standardized up-to-date gene descriptions. Our database will thus serve as a useful reference for the analysis of secondary metabolites. biosynthetic gene clusters.

open this section0.Interface overview

open this section1.Top page

open this section2.Compound list

open this section3.Cluster information

open this section4.CDS list

open this section5.CDS information

open this section6.Simple text search

open this section7.Advanced text search

open this section8.Module search

open this section9.BLAST search

open this section10.Category list

open this section11.Get sequence

open this section12.Data download

close this section13.PCR amplified sequences

We have extensively determined KS and A domain sequences in NBRC strains, and registered them here. The amplified sequences are listed in Phylogenetic tree. Users can open the lower by clicking each organism name. All strains registered here are available from NBRC. More details see here...

open this section14.Novelty chart

open this section15.PCR sequence BLAST

open this sectionXX.Other tips implemented in DoBISCUIT

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